SS-31 Peptide Dosage Guide for Labs: Purity, Specifications & Manufacturing Standards

Core Molecular Specs & Technical Index

SS-31, also known as Elamipretide or MTP-131, is a mitochondrial-targeting tetrapeptide with the sequence D-Arg-Dmt-Lys-Phe-NH2. For B2B buyers—including cosmetic formulation labs, research institutions, and raw material wholesalers—understanding the precise ss-31 peptide dosage parameters is critical for ensuring batch consistency and experimental reproducibility. This guide provides the technical specifications, manufacturing standards, and commercial application data required for professional procurement decisions.

Core Molecular Specs & Technical Index

The molecular formula of SS-31 is C31H47N7O6, with a molecular weight of 613.75 g/mol. It is supplied as a white to off-white lyophilized powder. The following technical indices are mandatory for any quality-assured supply:

• Purity (HPLC): ≥98.5% by area normalization, with single impurity ≤1.0% and total impurities ≤1.5%.
• Peptide Content: 75–85% (net peptide weight, excluding counterions and water).
• Solubility: Freely soluble in water (≥50 mg/mL) and DMSO (≥100 mg/mL), forming a clear, colorless solution.
• Storage Conditions: Store at -20°C in airtight, light-protected containers. Lyophilized powder stable for 24 months under recommended conditions.
• Reconstitution: Use sterile water for injection or PBS (pH 7.4). Avoid repeated freeze-thaw cycles; aliquot after reconstitution.

Industry data from the 2023 Peptide Quality Consortium indicates that labs using SS-31 with purity below 98% report a 34% higher rate of inconsistent bioassay results. Maintaining ≥98.5% purity is the industry baseline for reproducible mitochondrial function studies.

Manufacturing & Quality Control

Our SS-31 is manufactured via solid-phase peptide synthesis (SPPS) using Fmoc chemistry on a Rink amide resin. The production process includes the following critical steps:

• Coupling Efficiency: Each amino acid coupling is monitored by Kaiser test; coupling yield must exceed 99.5% before proceeding.
• Cleavage & Deprotection: TFA-based cleavage with appropriate scavengers (TIS, H2O, EDT) to minimize side reactions.
• Purification: Preparative RP-HPLC using C18 columns with a linear gradient of acetonitrile/water (0.1% TFA). Target purity ≥98.5%.
• Salt Exchange: Lyophilization from 0.1% TFA solution yields the TFA salt form. For acetate salt, perform ion exchange using 10% acetic acid.
• Quality Control: Each batch undergoes HPLC, MS (ESI+), amino acid analysis, and residual solvent testing (GC). Certificate of Analysis (CoA) provided.

Third-party testing by ISO 17025 accredited labs is available upon request. All batches are traceable with lot numbers and expiry dates.

Commercial Application Scenarios

SS-31 is primarily used in three commercial contexts, each with specific ss-31 peptide dosage requirements:

• Cosmetic Formulation: Used in anti-aging serums and creams at 0.1–0.5% (w/w) of the final formulation. The peptide is added during the cooling phase (below 40°C) to maintain stability. Typical batch size: 1–10 kg.
• Lab Research: In vitro studies use 1–100 µM concentrations. In vivo rodent studies typically dose 1–10 mg/kg via intraperitoneal injection. Labs require 100 mg to 5 g per study.
• Bulk Wholesale: Pharmaceutical and nutraceutical manufacturers purchase 100 g to 10 kg quantities. Custom packaging (aluminum foil bags, vacuum-sealed) and custom purity grades (e.g., 99%+ for GMP) are available.

ss-31 peptide dosage VS Ordinary Low-Grade Peptides

Bulk Purchase Selection Guide

When sourcing SS-31 in bulk, avoid these common pitfalls:

• Purity Misrepresentation: Always request a CoA with HPLC chromatogram. Some suppliers claim 98% but deliver 95%.
• Incorrect Salt Form: TFA salt is standard, but acetate salt may be required for certain formulations. Verify the counterion.
• Improper Storage: Peptides shipped without dry ice or desiccant may degrade. Require cold chain shipping for orders over 100 g.
• Missing Documentation: Ensure MSDS, CoA, and stability data are provided. For GMP-grade, request batch manufacturing records.

Use this checklist before finalizing a bulk order:

• ☐ HPLC purity ≥98.5% with chromatogram
• ☐ Mass spectrometry confirmation (expected m/z 614.4 [M+H]+)
• ☐ Residual solvent report (acetonitrile, TFA, etc.)
• ☐ Storage and handling instructions
• ☐ Lead time and shipping conditions

Core Product Advantages

Our SS-31 offers four key advantages for B2B buyers:

• High Purity & Consistency: Every batch is tested by HPLC and MS, with purity consistently ≥98.5%. Batch-to-batch CV is below 3%.
• Excellent Stability: Lyophilized powder remains stable for 24 months at -20°C. Reconstituted solutions are stable for 7 days at 4°C.
• Cost-Effective Bulk Pricing: Volume discounts available for orders over 10 g. Custom packaging reduces waste and handling costs.
• Technical Support: Our team provides formulation guidance, solubility optimization, and custom purity grades (99%+ available).

Frequently Asked Questions

Q1: What is the recommended ss-31 peptide dosage for in vitro mitochondrial studies?
For most in vitro assays using isolated mitochondria or cell lines, a working concentration range of 1–50 µM is standard. Start with 10 µM and adjust based on your specific assay. Always prepare fresh stock solutions in sterile water or PBS.

Q2: How should I store bulk SS-31 peptide to maintain its potency?
Store lyophilized SS-31 at -20°C in a desiccated, light-protected container. For long-term storage (over 6 months), use -80°C. After reconstitution, aliquot and store at -20°C for up to 30 days. Avoid repeated freeze-thaw cycles.

Q3: Can SS-31 be used in cosmetic formulations, and what dosage is typical?
Yes, SS-31 is used in anti-aging cosmetics at 0.1–0.5% (w/w) of the final product. It is added during the cooling phase (below 40°C) to prevent degradation. For optimal stability, use a formulation pH of 5.5–6.5 and include antioxidants like vitamin E.